Please note that the in vitro-cultured mast cells are derived from Buffy coats obtained with the informed consent of donors and in compliance with the Declaration of Helsinki and all other applicable regulations.

• The ordered amount of cells, which can be ordered in increments of 5 million cells, in complete culture medium.
• The required volume of culture medium to maintain the mast cells in culture for 14 days (storage 4°C). This culture medium MUST be used for culturing the cells.
• Batch certificate for Buffy coat with donor information
• Documentation:
  • SDS for Complete Culture Medium
  • Product sheet for Complete Culture Medium
  • Product sheet for in vitro-cultured mast cells
  • Donor lot release certificate

Connective tissue-type mast cells (CTMCs) are derived from CD34⁺ progenitors isolated from human peripheral blood and differentiated over 6–8 weeks in a defined combination of cytokines and growth factors. Quality control (QC) includes characterization of mast cells using flow cytometry to assess expression of CD117 and  FcεRI. Additionally, degranulation assays are conducted to evaluate the functional response of the cells by stimulating them with anti-IgE, triggering the release of intracellular granules. Degranulation is measured using a  ß-hexosaminidase assay or CD63 expression via flow cytometry.

Shipping Format: Room temperature

Receipt Instructions: Upon receipt, cells should be centrifuged and transferred to culture using the Complete Culture Medium that is provided with the shipment of the cells. The cells should be cultured for 5-7 days prior to using for testing and used within 2 weeks of receipt.

This product is intended for laboratory research use only. It is not intended for any human or animal therapeutic use, diagnostic purposes, or any form of human or animal consumption. Ensure all cell handling is performed under strict aseptic conditions under biosafety level 2 conditions to prevent contamination.

Biosafety Level: BSL-2, handle as potentially hazardous material.

Personal Protective Equipment (PPE): Wear appropriate protective eyewear, gloves, and a lab coat while handling the cells and Complete Culture Medium.

Potential Hazards: Human primary cells may carry pathogens; therefore, standard precautions for handling biological materials must be followed.

Modifications to orders can only be accepted within 24 hours of placement and will incur a 10% fee.